The light source of the microscope cannot perfectly uniformly illuminate the surface of an object. When stitching a large image from a set of smaller ones, these uneven lighting lead to a bad result - the stitching seams become visible. The flat-field correction tool allows you to compensate for uneven lighting.
The following describes the process of collecting calibration data for the flat-field correction. You need to do it ones, after that it can be used in stitching process.
Set the object perpendicular to the optical axis of the lens and focus on the surface of the object. Also it is necessary that the object occupies the entire field of view:
Push the Start lighting calibration button: