Place some flat object with texture under the microscope. In should be all in focus, i.e. perpendicular to the camera.
The application should look like that: 

The plugin will tell you if everything it's ok or recommend to perform calibration if something is wrong with the vertical disparity. If there is too few information the plug-in will also tell you about it.
After checking the application should look like some of the screenshots below:
| Status | Screenshot example | Required actions |
|---|---|---|
| Good result | ![]() | Continue working, nothing is required |
| Fair result | ![]() | Continue working, but re-calibration is recommended |
| Bad result | ![]() | Re-calibration is required. |
| The result with too few data | ![]() | The object is not suitable for checking procedure, please change the object (paper, coin, etc) |