The light source of the microscope cannot perfectly uniformly illuminate the surface of an object. When stitching a large image from a set of smaller ones (see here for details), these uneven lighting lead to a bad result - the stitching seams become visible. The flat-field correction tool allows you to compensate for uneven lighting
The GUI for the Flat-Field Correction tool is located in the Image Stitching panel (in the right panel of the Processes tab)
The status of supporting the flat-field correction for current zoom. Determines if calibration data for compensation is available
Supported | Not supported |
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The following describes the process of collecting calibration data for the flat-field correction.
Set the object perpendicular to the optical axis of the lens and focus on the surface of the object. Also it is necessary that the object occupies the entire flat of view when minimum zoom
Push the Start lighting calibration button